Influence of genetic polymorphisms on oral health-related quality of life after root canal treatment

Abstract To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT). Methods: The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT. Saliva samples were collected as a source of genomic DNA. Genetic polymorphisms were genotyped by Real-Time PCR using the Taqman method. Univariate and Multivariate analyses were used (p<0.05). Results: A significant difference was observed for the polymorphism rs2297518 in the NOS2 gene in functional limitation in the codominant (p=0.037) and recessive (p=0.001) models; in the physical pain (p<0.001 in both models); in psychological discomfort (p<0.001 in both models); in physical disability (p<0.001 in both models) and in psychological disability (p<0.001 in both models). Polymorphisms in the SOCS1 gene, in the recessive model, rs33977706 (p=0.045) and rs243327 (p=0.019), influenced the OHRQoL in the psychological discomfort domain. Conclusions: Polymorphisms in NOS2 and SOCS1 genes influenced the OHRQoL of patients undergoing RCT.

Resumo Avaliar o impacto de polimorfismos genéticos em interleucinas (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), óxido nítrico (NOS2 rs2779249, rs2897518) e supressor da sinalização de citocinas (SOCS1 rs243327, rs33977706) na qualidade de vida relacionada à saúde bucal (QVRSB) de pacientes submetidos a tratamento endodôntico (TE). Métodos: A amostra foi composta por 108 participantes, que apresentavam dentes unirradiculares com lesão periapical assintomática. O impacto da QVRSB foi registrado usando o Oral Health Impact Profile (OHIP-14) antes, sete e 30 dias após o TE. Amostras de saliva foram coletadas como fonte de DNA genômico. Os polimorfismos genéticos foram genotipados por PCR em tempo real usando o método Taqman. Análises univariadas e multivariadas foram utilizadas (p<0,05). Resultados: Observou-se diferença significativa para o polimorfismo rs2297518 no gene NOS2 na limitação funcional nos modelos codominante (p=0,037) e recessivo (p=0,001); na dor física (p<0,001 em ambos os modelos); no desconforto psicológico (p<0,001 em ambos os modelos); na deficiência física (p<0,001 em ambos os modelos) e na deficiência psicológica (p<0,001 em ambos os modelos). Polimorfismos no gene SOCS1, no modelo recessivo, rs33977706 (p=0,045) e rs243327 (p=0,019), influenciaram a QVRSB no domínio desconforto psicológico. Conclusões: Polimorfismos nos genes NOS2 e SOCS1 influenciaram a QVRSB de pacientes submetidos a TE.

Genetic polymorphisms in TNF-α as a potential biomarker for oral health-related quality of life in children

Abstract: This cross-sectional study aimed to assess if genetic polymorphisms in TNF- α are associated with a negative impact on Oral Health-Related Quality of Life (OHRQoL) in children with dental caries. A total of 307 pairs of parents/caregivers and children aged two to five years were selected. The children were clinically evaluated and classified according to caries experience and severity of active caries. The Brazilian Portuguese version of the Early Childhood Oral Health Impact Scale (ECOHIS) was used to assess OHRQoL. Genotyping analysis of genetic polymorphisms in TNF- α (rs1799724, rs1799964, and rs1800629) was performed using real-time polymerase chain reaction. In the recessive model, children with the CC genotype of TNF-α (rs1799964) had a significantly high chance of poor OHRQoL in the symptom domain (pain), in both the caries experience (p = 0.045) and the high-severity active caries phenotypes (p = 0.033) (Mann-Whitney U test). It was concluded that genetic polymorphisms in TNF-α are associated with OHRQoL related to the symptom domain (pain), suggesting that TNF-α could be used as a potential biomarker for OHRQoL. Understanding the genetic aspects associated with OHRQoL will allow the early identification of patients with OHRQoL disparities and provide personalized healthcare.

Perfil Epidemiológico dos Traumatismos Dentários em Crianças e Adolescentes no Brasil / Epidemiological Profile of Dental Trauma in Children and Adolescents in Brazil

O traumatismo dentário - TD é considerado, pela Organização Mundial de Saúde ? OMS um problema de saúde pública que apresenta alta prevalência e gera impacto negativo na qualidade de vida relacionada à saúde bucal de crianças/adolescentes e familiares com sequelas biológicas, socioeconômicas, psicológicas e comportamentais. Assim, o presente estudo objetivou realizar uma revisão critica da literatura sobre os estudos que avaliam o perfil epidemiológico do TD em crianças e adolescentes, mapeando a distribuição desses estudos no Brasil. A busca na literatura foi feita utilizando os descritores: traumatismos dentários, criança, adolescente, e epidemiologia, nas Bases Medline, Lilacs, SciELO, BBO e no Google Acadêmico. Os mesmos termos em inglês foram utilizados no Pubmed. De 248 artigos encontrados inicialmente, foram removidas as duplicatas e aplicados os critérios de inclusão e exclusão, selecionando-se 53 artigos. Observou-se que há muitos estudos sobre a prevalência dos TD desenvolvidos no Brasil durante os últimos anos. No entanto, os estudos estão concentrados em algumas regiões específicas como Rio Grande do Sul, Santa Catarina, Minas Gerais e São Paulo. A prevalência de TD em dentição decídua foi de 9,4% e 62,1%, e permanente variou de 8,0% a 58,6%. Os fatores como gênero, sobressaliência acentuada e cobertura labial inadequada foram frequentemente associados ao TD em crianças e adolescentes. Baseado nos levantamentos epidemiológicos dos artigos selecionados neste trabalho, conclui-se que, devido à concentração das pesquisas em alguns centros, não há um perfil representativo da população brasileira e os dados compilados dos estudos devem ser comparados com cautela, devido à grande diferença metodológica existente nos estudos.

Traumatic dental injury (TDI) is considered by the World Health Organization as a public health problem with high prevalence, which generates negative impact on oral health related to quality of life of children / adolescents and their families, causing biological, socioeconomic, psychological and behavioral sequelae. The present study aimed to conduct a critical review of the literature on studies assessing the epidemiological profile of the TDI in children and adolescents, mapping the distribution of these studies in Brazil. The search was performed using the descriptors ?dental trauma, children, adolescents, epidemiology and Brazil?, in the Medline, Lilacs, SCiELO, BBO, Google Scholar, and Pubmed. Initially, 248 articles were found. After removing the duplicates and applying the inclusion and exclusion criteria, 53 articles were selected. It has been observed that there are many studies of prevalence of TDI developed in recent years in Brazil. However, these studies are concentrated in some specific areas such as Rio Grande do Sul, Santa Catarina, Minas Gerais, and São Paulo. The prevalence of TDI in the primary dentition was 9.4% and 62.1%, ranging from 8.0% to 58.6% in the permanent dentition. Factors such as gender, increased overjet and inadequate lip coverage were often associated with TDI in children and adolescents. Based on the epidemiological surveys of the articles selected in this study, we concluded that there is no representative profile of the Brazilian population due to the concentration of research in some centers, thus data should be compared with caution due to the large methodological difference between the studies.